High resolution mapping of the tumor microenvironment using integrated single-cell, spatial and in situ analysis
Articolo
Data di Pubblicazione:
2023
Abstract:
Single-cell and spatial technologies that profile gene expression across a whole tissue are revolutionizing the resolution of molecular states in clinical samples. Current commercially available technologies provide whole transcriptome single-cell, whole transcriptome spatial, or targeted in situ gene expression analysis. Here, we combine these technologies to explore tissue heterogeneity in large, FFPE human breast cancer sections. This integrative approach allowed us to explore molecular differences that exist between distinct tumor regions and to identify biomarkers involved in the progression towards invasive carcinoma. Further, we study cell neighborhoods and identify rare boundary cells that sit at the critical myoepithelial border confining the spread of malignant cells. Here, we demonstrate that each technology alone provides information about molecular signatures relevant to understanding cancer heterogeneity; however, it is the integration of these technologies that leads to deeper insights, ushering in discoveries that will progress oncology research and the development of diagnostics and therapeutics.
Tipologia CRIS:
1.1 Articolo in rivista
Elenco autori:
Amanda, Janesick; Robert, Shelansky; Andrew, D Gottscho; Florian, Wagner; Stephen, R Williams; Morgane, Rouault; Ghezal, Beliakoff; Carolyn, A Morrison; Michelli, F Oliveira; Jordan, T Sicherman; Andrew, Kohlway; Jawad, Abousoud; Tingsheng Yu, Drennon; Seayar, H Mohabbat; 10x Development, Teams; Jawad, Abousoud; Francis, Aguisanda; Matt, Alexander; Maria, Alexis; Pratomo, Alimsijah; Morris
Allison, ; Aditya, Aman; Joseph, Aman; Zaid, Ammari; Naishitha, Anaparthy; Eric, Anderson; Courtney, Anderson; Melissa, Ando; Joey, Arthur; Adam, Azarchs; Navi, Bains; Naveed, Bakh; Alexandru, Bardasu; Carlos, Barraza; Ashley, Basco; David, Batey; Florian, Baumgartner; Bava, F; Kamila, Belhocine; Jason, Bell; Julio, Beltran; Alex, Ben; Zachary, Bent; Casey, (Jon)
Berridge; Rajiv, Bharadwaj; Anton, Bjorninen; Kirk, Blackmoore; Octavian, Bloju; Lorita, Boghospor; Jeff, Bolland; Raj, Borade; Erik, Borgstrom; Sebastian Le, Bras; Christian, Broms; Phillip, Brooks; Brenden, Brown; Noel Harris, Brown; Elizabeth, Buckley; Patricia, Buenbrazo; Ratha, Bun; Diana
Burkart-Waco, ; Jim, Burrows; Michele, Busby; Janae, Bustos; Michele, Caceres; Matthew, Cai; Xiaoyang (Frank), Cai; Michael, Campbell; Saranya, Canchi; Joshua, Cataldo; Emily, Chan; Rena
Chan, ; Alan, Chang; Evelyn, Chang; Ella Hsinwen, Chang; Sharmila, Chatterjee; Sidharth
Chaturvedi, ; Mitu, Chaudhary; James, Chell; Kevin, Chen; Sarah, Chen; Hong, Chen; Jui-Szu
Chen, ; Sharon, Chen; Brian, Cheng; Jennifer, Cheung; Jennifer, Chew; Jun Ding, Chiang; Hariharasudhan, Chirra; Cheyenne, Christopherson; Sonya, Clark; Brynn, Claypoole; Justin, Costa; Julia, Cowen; David, Cox; Francis, Cui; Abbey, Cutchin; Aos, Dabbagh; Smritee, Dadhwal; Eileen
Dalin, ; Aldo, Deamicis; Filip, Defoort; Evan, Dejarnette; Joshua, Delaney; Nigel, Delaney; Loruhama, Delgado-Rivera; Maurizio, Depoli; Abou, Diop; Rhyan, Dockter; Keri, Dockter; Sultan
Doganay, ; Jason, Downing; Tyler, Drake; Shoshoni, Droz; Narek, Dshkhunyan; Jens, Durruthy
Durruthy; Carine, Edder; Peter, Edge; Aaron, Ellis; Emre, Erhan; James, Erhard; Cedric, Espenel; Danielle, Estes; Eric, Evje; Navid, Farahani; Reynaldo, Farias; Dina, Finan; Melanie, Freeman; Danny, Freitas; Tristan, French; Meghan, Frey; Alex, Gagnon; Caroline, Gallant; Abigail, Gallegos; Christina, Galonska; Karthik, Ganapathy; Richard, Gantt; Jason, Gao; Kevin, Gilmartin; Ryan
Gilmore, ; Gabriele, Girelli; Andreas, Girgensohn; Michael, Glazer; Shalini, Gohil; Sumanth
Gollapudi, ; Qiang, Gong; Brandon, Gordon; Erica, Graziosa; Yang, Gu; Josh, Gu; Zhenping, Guan; Zhengping, Guan; Christopher, Guido; Madhu, Gundapuneni; Vaibhav, Gupta; Rashmi, Gupta; Lauren, Gutgesell; Daniel, Gyllborg; Lila, Haba; Katherine, Habeck; Andrew, Haling; Katrín
Halldórsdóttir, ; Andrej, Hartnett; Ryo, Hatori; Eileen, He; Andrew, Heiberg; Robert, Henley; Janine
Hensel, ; Lance, Hepler; Alexander, Hermes; Javier, Hernandez; Iván, Hernández; Jack, Herrera; Jill
Herschleb, ; Andrew, Hill; Ben, Hindson; Michael, Hirte; Khoa, Hoang; David, Hoffman; Catie
Hollyer, ; Eric, Holt; Babak, Honaryar; Kc, Hong; Daniel, Horvath; Jennifer, Hsu; Alice, Huang; Winnie, Hui; Kim Xuan, Huynh; Hayato, Ikoma; Moussa, Iskandar; Eswar, Iyer; Shaun, Jackman; Sunil, Jagannath; Samir, Jain; Thomas, Johnson; Tom, Johnson; Guy, Joseph; Aleksandra, Jurek; Aarushi, Kalaimani; Govinda, Kamath; Lily Blanche, Kameny; Ivar, Karam; Rishabh, Kasliwal; Layla
Katiraee, ; Anna-Maria, Katsori; Matthew, Keeshen; Andrew, Kessler; Tousif, Khan; Aliyya, Khan; Hanyoup, Kim; Albert, Kim; James, Kim; Minji, Kim; Sugyeom, Kim; Alex, Kindwall; Naga, Sudha
Kodavatikanti; Andrew, Kohlway; Sutheng, Kok; Sukhmal, Kommidi; Nikola, Kondov; Aishwarya
Konnur, ; Jason, Koth; Maria, Kourbatov; Joseph, Kovac; Sreenath, Krishnan; Benjamin, Ku; Jing
Kuang, ; Malte, Kuhnemund; Divya Anjan, Kumar; Vijay, Kumar; Josy, Kuriakose; Sural, Labha; Yves, Lacroix; Karen, Lai; Jana, Lalakova; Tim, Lam; Du Linh, Lam;
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