Qualitative and semi-quantitative phytochemical analysis on the seeds of a new Nigella sativa L. population exemplar from Iran

In this work, the first phytochemical analysis on the seeds of Nigella sativa L. collected from a new population in Iran is reported. The study was carried out by means of classical column chromatography and spectroscopic techniques and evidenced the presence of twenty-six compound divided into six different classes of natural compounds. In particular, compounds (3–4) have been reported in the family for the first time. In addition to this, the first NMR semi-quantitative analysis on their most polar metabolites content was reported. The chemotaxonomic and pharmacological aspects of all the identified compounds were discussed.